kapilarna elektroforeza

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capillary electrophoresis

Također vas mogu zanimati sljedeći pojmovi / You may also be interested in the following terms:

  1. kapilara - capillaries, capillary
  2. kapilaran - capillary
  3. kapilarna barijera - capilar barriers
  4. kapilarna elektroforeza - capillary electrophoresis
  5. kapilarnost - capillarity

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Soki@posao (dosadno mi), (14.01.2009)
Capillary electrophoresis (CE), also known as capillary zone electrophoresis (CZE), can be used to separate ionic species by their charge and frictional forces. In traditional electrophoresis, electrically charged analytes move in a conductive liquid medium under the influence of an electric field. Introduced in the 1960s, the technique of capillary electrophoresis (CE) was designed to separate species based on their size to charge ratio in the interior of a small capillary filled with an electrolyte. While its use has been sporadic, CE offers unparalleled resolution and selectivity allowing for separation of analytes with very little physical difference. Efficiencies of millions of plates are routinely reported. Once thought impossible, separation of large proteins differing in only one amino acid (ie. D-Lysine substituted for L-Lysine) and even an isotopic separation of 14N and 15N ammonium hydroxide have been reported. No other technique has shown such powerful selectivity with the ability for extremely high sensitivity. As few as 6 molecules of a substance have been separated and detected with the help of laser-induced fluorescence (LIF).

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